Composite
Part:BBa_K914014:Design
Designed by: Dylan Iverson Group: iGEM12_Paris_Bettencourt (2012-10-25)
Pbad/AraC RBS LacI[LVA(-)] Plac GFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1247
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1187
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3099
Illegal SapI site found at 961
Design Notes
LVA tag was removed from LacI by PCR. The delay was not functional with fast-degrading LacI.
Source
Regristry kits